1q16 Summary

pdbe.org/1q16
spacer

Crystal structure of Nitrate Reductase A, NarGHI, from Escherichia coli

The structure was published by Bertero, M.G., Rothery, R.A., Palak, M., et al., Blasco, F., Weiner, J.H., and Strynadka, N.C.J., in 2003 in a paper entitled "Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.9 Å and deposited in 2003.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Respiratory nitrate reductase 1 alpha chain, Respiratory nitrate reductase 1 beta chain, and Respiratory nitrate reductase 1 gamma chain.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Respiratory nitrate reductase 1 alpha chain P09152 (1-1247) (NARG_ECOLI)search Escherichia coli K-12search 100% 1247 99%
B Respiratory nitrate reductase 1 beta chain P11349 (1-512) (NARH_ECOLI)search Escherichia coli K-12search 99% 512 99%
C Respiratory nitrate reductase 1 gamma chain P11350 (1-225) (NARI_ECOLI)search Escherichia coli K-12search 100% 225 99%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P09152 (1 - 1247) Respiratory nitrate reductase 1 alpha chain Escherichia coli
P11349 (1 - 512) Respiratory nitrate reductase 1 beta chain Escherichia coli
P11350 (1 - 225) Respiratory nitrate reductase 1 gamma chain Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P09152) Formate dehydrogenase/DMSO reductase, C-terminal domainsearch, Formate dehydrogenase/DMSO reductase, domains 1-3search nitrate reductase tailsearch, ADC-like domainssearch, Rossmann foldsearch, Dimethylsulfoxide Reductase, domain 2search, Barwin-like endoglucanasessearch PF00384: Molybdopterin oxidoreductasesearch, PF01568: Molydopterin dinucleotide binding domainsearch, PF14710: Respiratory nitrate reductase alpha N-terminalsearch
B (P11349) Ferredoxin domains from multidomain proteinssearch Alpha-Beta Plaitssearch, nitrate reductase domain likesearch PF13247: 4Fe-4S dicluster domainsearch, PF14711: Respiratory nitrate reductase beta C-terminalsearch
C (P11350) Respiratory nitrate reductase 1 gamma chainsearch Transmembrane di-heme cytochromes, Chain Csearch PF02665: Nitrate reductase gamma subunitsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A (P09152) protein bindingsearch nitrate reductase activitysearch electron carrier activitysearch oxidoreductase activitysearch oxidoreductase activity, acting on NAD(P)Hsearch molybdenum ion bindingsearch metal ion bindingsearch iron-sulfur cluster bindingsearch 4 iron, 4 sulfur cluster bindingsearch plasma membranesearch nitrate reductase complexsearch membranesearch intrinsic component of membranesearch anaerobic respirationsearch cytochrome complex assemblysearch nitrate metabolic processsearch nitrate assimilationsearch oxidation-reduction processsearch
B (P11349) protein bindingsearch nitrate reductase activitysearch electron carrier activitysearch oxidoreductase activitysearch metal ion bindingsearch iron-sulfur cluster bindingsearch 3 iron, 4 sulfur cluster bindingsearch 4 iron, 4 sulfur cluster bindingsearch cytoplasmsearch plasma membranesearch nitrate reductase complexsearch membranesearch intrinsic component of membranesearch anaerobic respirationsearch cytochrome complex assemblysearch nitrate metabolic processsearch nitrate assimilationsearch oxidation-reduction processsearch
C (P11350) nitrate reductase activitysearch oxidoreductase activitysearch metal ion bindingsearch plasma membranesearch nitrate reductase complexsearch membranesearch integral component of membranesearch intrinsic component of membranesearch anaerobic respirationsearch cytochrome complex assemblysearch nitrate assimilationsearch oxidation-reduction processsearch

Chain InterPro annotation
A Nitrate reductase, alpha subunitsearch Molybdopterin oxidoreductase, prokaryotic, conserved sitesearch Molybdopterin oxidoreductasesearch Molybdopterin dinucleotide-binding domainsearch Molybdopterin oxidoreductase, 4Fe-4S domainsearch Aspartate decarboxylase-like domainsearch Molybdopterin oxidoreductase, molybdopterin cofactor binding sitesearch Nitrate reductase, alpha subunit, N-terminalsearch
B Nitrate reductase, beta subunitsearch 4Fe-4S ferredoxin-type, iron-sulphur binding domainsearch Respiratory nitrate reductase beta, C-terminalsearch
C Nitrate reductase, gamma subunitsearch NarG-like domainsearch