› 1pwh

X-ray diffraction
2.6Å resolution

Rat Liver L-Serine Dehydratase- Complex with PYRIDOXYL-(O-METHYL-SERINE)-5-MONOPHOSPHATE

Released:
DOI: 10.2210/pdb1pwh/pdb
PDB entry 1pwh coloured by chain, front view.
PDB entry 1pwh coloured by chain, side view.
PDB entry 1pwh coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of serine dehydratase from rat liver.
Yamada T, Komoto J, Takata Y, Ogawa H, Pitot HC, Takusagawa F
Biochemistry 42 12854-65 (2003)
PMID: 14596599

Function and Biology Details

Reactions catalysed: 
(1a) L-serine = 2-aminoprop-2-enoate + H(2)O
(1a) L-threonine = 2-aminobut-2-enoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140618 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-serine dehydratase/L-threonine deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 34.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09367 (Residues: 1-363; Coverage: 90%)
Gene name: Sds
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand K 4 x K
Ligand PLV 4 x PLV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 62.241Å b: 109.262Å c: 98.94Å
α: 90° β: 91.66° γ: 90°
R-values:
R R work R free
0.26 0.23 0.26
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
5 more

7 mentions without citation

The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding.
Smith et al. (2010)
6 more