1pk0 Summary

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Crystal Structure of the EF3-CaM complexed with PMEApp

The structure was published by Shen, Y., Zhukovskaya, N.L., Zimmer, M.I., et al., Wang, C.R., Gibbs, C.S., and Tang, W.J., in 2004 in a paper entitled "Selective inhibition of anthrax edema factor by adefovir, a drug for chronic hepatitis B virus infection." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.3 Å and deposited in 2003.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (292-798) (CYAA_BACAN)search Bacillus anthracissearch 96% 507 99%
B Calmodulin-sensitive adenylate cyclase P40136 (292-798) (CYAA_BACAN)search Bacillus anthracissearch 96% 507 99%
C Calmodulin-sensitive adenylate cyclase P40136 (292-798) (CYAA_BACAN)search Bacillus anthracissearch 96% 507 99%
D Calmodulin P62158 (2-148) (CALM_HUMAN)search Homo sapienssearch 97% 147 97%
E Calmodulin P62158 (2-148) (CALM_HUMAN)search Homo sapienssearch 97% 147 97%
F Calmodulin P62158 (2-148) (CALM_HUMAN)search Homo sapienssearch 97% 147 97%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (292 - 798) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (2 - 148) Calmodulin Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C (P40136) Adenylylcyclase toxin (the edema factor)search Adenylylcyclase toxin superfamilysearch PF03497: Anthrax toxin LF subunitsearch
D, E, F (P62158) Calmodulin-likesearch EF-handsearch PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B, C (P40136) calcium- and calmodulin-responsive adenylate cyclase activitysearch pathogenesissearch extracellular regionsearch
D, E, F (P62158) calcium ion bindingsearch protein serine/threonine kinase activator activitysearch protein bindingsearch calcium-dependent protein bindingsearch protein phosphatase activator activitysearch nitric-oxide synthase regulator activitysearch metal ion bindingsearch phospholipase bindingsearch protein domain specific bindingsearch thioesterase bindingsearch protein N-terminus bindingsearch adenylate cyclase bindingsearch phosphatidylinositol 3-kinase bindingsearch ion channel bindingsearch enzyme regulator activitysearch N-terminal myristoylation domain bindingsearch type 3 metabotropic glutamate receptor bindingsearch nitric-oxide synthase bindingsearch protein kinase bindingsearch titin bindingsearch inositol phosphate metabolic processsearch regulation of heart ratesearch negative regulation of peptidyl-threonine phosphorylationsearch platelet activationsearch neurotrophin TRK receptor signaling pathwaysearch positive regulation of protein serine/threonine kinase activitysearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch positive regulation of peptidyl-threonine phosphorylationsearch membrane organizationsearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch fibroblast growth factor receptor signaling pathwaysearch regulation of high voltage-gated calcium channel activitysearch calcium-mediated signalingsearch synaptic transmissionsearch response to corticosteronesearch glucose metabolic processsearch platelet degranulationsearch epidermal growth factor receptor signaling pathwaysearch phototransduction, visible lightsearch regulation of nitric-oxide synthase activitysearch blood coagulationsearch regulation of ryanodine-sensitive calcium-release channel activitysearch substantia nigra developmentsearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch positive regulation of protein autophosphorylationsearch response to amphetaminesearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch signal transductionsearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch Fc-epsilon receptor signaling pathwaysearch positive regulation of protein dephosphorylationsearch regulation of cardiac muscle contractionsearch response to calcium ionsearch positive regulation of cyclic nucleotide metabolic processsearch rhodopsin mediated signaling pathwaysearch G-protein coupled receptor signaling pathwaysearch regulation of rhodopsin mediated signaling pathwaysearch nitric oxide metabolic processsearch positive regulation of phosphoprotein phosphatase activitysearch positive regulation of nitric-oxide synthase activitysearch carbohydrate metabolic processsearch glycogen catabolic processsearch activation of adenylate cyclase activitysearch small molecule metabolic processsearch muscle contractionsearch innate immune responsesearch detection of calcium ionsearch regulation of cytokinesissearch activation of phospholipase C activitysearch spindle polesearch cytosolsearch nucleussearch plasma membranesearch growth conesearch extracellular vesicular exosomesearch nucleoplasmsearch extracellular regionsearch cytoplasmsearch vesiclesearch sarcomeresearch cytoskeletonsearch centrosomesearch neuron projectionsearch calcium channel complexsearch spindle microtubulesearch

Chain InterPro annotation
A, B, C Anthrax toxin, edema factor, centralsearch
D, E, F EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch