› 1pjq

X-ray diffraction
2.21Å resolution

Structure and function of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis

Released:
DOI: 10.2210/pdb1pjq/pdb
PDB entry 1pjq coloured by chain, front view.
PDB entry 1pjq coloured by chain, side view.
PDB entry 1pjq coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis.
Stroupe ME, Leech HK, Daniels DS, Warren MJ, Getzoff ED
Nat Struct Biol 10 1064-73 (2003)
PMID: 14595395

Function and Biology Details

Reactions catalysed: 
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH
(1a) S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1
Siroheme + 2 H(+) = sirohydrochlorin + Fe(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
2 bound ligands:
Ligand ACT 3 x ACT
Ligand PGE 1 x PGE
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 60.734Å b: 121.493Å c: 130.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.235 0.273
Expression system: Salmonella enterica

Quick links

Citations

7 review citations

Architectures, mechanisms and molecular evolution of natural product methyltransferases.
Liscombe et al. (2012)
6 more

8 mentions without citation

Natural history of S-adenosylmethionine-binding proteins.
Kozbial et al. (2005)
7 more