› 1pey

X-ray diffraction
2.25Å resolution

Crystal structure of the Response Regulator Spo0F complexed with Mn2+

Released:
DOI: 10.2210/pdb1pey/pdb
PDB entry 1pey coloured by chain, front view.
PDB entry 1pey coloured by chain, side view.
PDB entry 1pey coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Metals in the sporulation phosphorelay: manganese binding by the response regulator Spo0F.
Mukhopadhyay D, Sen U, Zapf J, Varughese KI
Acta Crystallogr D Biol Crystallogr 60 638-45 (2004)
PMID: 15039551

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sporulation initiation phosphotransferase F Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 124 amino acids
Theoretical weight: 14.17 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06628 (Residues: 1-124; Coverage: 100%)
Gene names: BSU37130, spo0F
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 103.128Å b: 103.128Å c: 83.641Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.225 0.258
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

A combination of glycerol and manganese promotes biofilm formation in Bacillus subtilis via histidine kinase KinD signaling.
Shemesh et al. (2013)
5 more

6 mentions without citation

Identification of direct residue contacts in protein-protein interaction by message passing.
Weigt et al. (2009)
5 more