spacer Fitting of gp5 in the cryoEM reconstruction of the bacteriophage T4 baseplate
Primary citation
Title Three-dimensional structure of the bacteriophage T4 baseplate
Authors Kostyuchenko, V.A.search; Leiman, P.G.search; Chipman, P.R.search; Kanamaru, S.search; van Raaij, M.J.search; Arisaka, F.search; Mesyanzhinov, V.V.search; Rossmann, M.G.search
Journal NAT.STRUCT.BIOL.search vol:10, pag:688-693 (2003), Identifiers: PubMed ID (12923574)search DOI (10.1038/nsb970)
Abstract The baseplate of bacteriophage T4 is a multiprotein molecular machine that controls host cell recognition, attachment, tail sheath contraction and viral DNA ejection. We report here the three-dimensional structure of the baseplate-tail tube complex determined to a resolution of 12 A by cryoelectron microscopy. The baseplate has a six-fold symmetric, dome-like structure approximately 520 A in diameter and approximately 270 A long, assembled around a central hub. A 940 A-long and 96 A-diameter tail tube, coaxial with the hub, is connected to the top of the baseplate. At the center of the dome is a needle-like structure that was previously identified as a cell puncturing device. We have identified the locations of six proteins with known atomic structures, and established the position and shape of several other baseplate proteins. The baseplate structure suggests a mechanism of baseplate triggering and structural transition during the initial stages of T4 infection.
MeSH terms Bacteriophage T4search, Cryoelectron Microscopysearch, Image Processingsearch, Computer-Assistedsearch, Microscopysearch, Electronsearch, Modelssearch, Molecularsearch, Protein Structuresearch, Tertiarysearch
Other entries described in this publication 1pdm, 1pdf, 1pdj, 1pdi, 1pdp
Secondary citations
Title Structure of the cell-puncturing device of bacteriophage T4
Authors Kanamaru, S.search; Leiman, P.G.search; Kostyuchenko, V.A.search; Chipman, P.R.search; Mesyanzhinov, V.V.search; Arisaka, F.search; Rossmann, M.G.search
Journal NATUREsearch vol:415, pag:553-557 (2002)
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