› 1pcx

X-ray diffraction
2.5Å resolution

Crystal structure of the COPII coat subunit, Sec24, complexed with a peptide from the SNARE protein Bet1

Released:
DOI: 10.2210/pdb1pcx/pdb
PDB entry 1pcx coloured by chain, front view.
PDB entry 1pcx coloured by chain, side view.
PDB entry 1pcx coloured by chain, top view.
Source organisms:
Primary publication:
SNARE selectivity of the COPII coat.
Mossessova E, Bickford LC, Goldberg J
Cell 114 483-95 (2003)
PMID: 12941276

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149803 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein transport protein SEC24 Chain: A
Molecule details ›
Chain: A
Length: 810 amino acids
Theoretical weight: 90.84 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40482 (Residues: 117-926; Coverage: 88%)
Gene names: ANU1, SEC24, YIL109C
Sequence domains:
Structure domains:
Protein transport protein BET1 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 834 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P22804 (Residues: 51-58; Coverage: 6%)
Gene names: BET1, SLY12, YIA4C, YIL004C

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 94.93Å b: 94.93Å c: 198.574Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.259
Expression system: Not provided

Quick links

Citations

71 review citations

SNAREs--engines for membrane fusion.
Jahn et al. (2006)
70 more

1 mention without citation

Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.
Hutchings et al. (2021)