› 1pbg

X-ray diffraction
2.3Å resolution

THE THREE-DIMENSIONAL STRUCTURE OF 6-PHOSPHO-BETA GALACTOSIDASE FROM LACTOCOCCUS LACTIS

Released:
DOI: 10.2210/pdb1pbg/pdb
PDB entry 1pbg coloured by chain, front view.
PDB entry 1pbg coloured by chain, side view.
PDB entry 1pbg coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
The three-dimensional structure of 6-phospho-beta-galactosidase from Lactococcus lactis.
Wiesmann C, Beste G, Hengstenberg W, Schulz GE
Structure 3 961-8 (1995)
PMID: 8535789

Function and Biology Details

Reaction catalysed: 
A 6-phospho-beta-D-galactoside + H(2)O = 6-phospho-D-galactose + an alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-phospho-beta-galactosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 54.14 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P11546 (Residues: 1-468; Coverage: 100%)
Gene name: lacG
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 60.5Å b: 83.2Å c: 105.7Å
α: 90° β: 93.7° γ: 90°
R-values:
R R work R free
0.164 0.164 0.236
Expression system: Escherichia coli

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Citations

2 review citations

Mutagenesis of glycosidases.
Ly et al. (1999)
1 more

5 mentions without citation

Structural basis for thermostability of beta-glycosidase from the thermophilic eubacterium Thermus nonproteolyticus HG102.
Wang et al. (2003)
4 more