› 1p9r

X-ray diffraction
2.5Å resolution

Crystal Structure of Vibrio cholerae putative NTPase EpsE

Released:
DOI: 10.2210/pdb1p9r/pdb
PDB entry 1p9r coloured by chain, front view.
PDB entry 1p9r coloured by chain, side view.
PDB entry 1p9r coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae.
Robien MA, Krumm BE, Sandkvist M, Hol WG
J Mol Biol 333 657-74 (2003)
PMID: 14556751

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II secretion system ATPase E Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.12 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: P37093 (Residues: 91-498; Coverage: 81%)
Gene names: VC_2732, epsE
Sequence domains: Type II/IV secretion system protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P6122
Unit cell:
a: 103.611Å b: 103.611Å c: 166.261Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.265
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Biogenesis, architecture, and function of bacterial type IV secretion systems.
Christie et al. (2005)
21 more

7 mentions without citation

Structural insights into the Type II secretion nanomachine.
McLaughlin et al. (2012)
6 more