› 1p9b

X-ray diffraction
2Å resolution

Structure of fully ligated Adenylosuccinate synthetase from Plasmodium falciparum

Released:
DOI: 10.2210/pdb1p9b/pdb
PDB entry 1p9b coloured by chain, front view.
PDB entry 1p9b coloured by chain, side view.
PDB entry 1p9b coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Crystal structure of fully ligated adenylosuccinate synthetase from Plasmodium falciparum.
Eaazhisai K, Jayalakshmi R, Gayathri P, Anand RP, Sumathy K, Balaram H, Murthy MR
J Mol Biol 335 1251-64 (2004)
PMID: 14729341

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase Chain: A
Molecule details ›
Chain: A
Length: 442 amino acids
Theoretical weight: 50.09 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9U8D3 (Residues: 3-442; Coverage: 100%)
Gene name: Adss
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand NO3 1 x NO3
Ligand IMO 1 x IMO
Ligand HDA 1 x HDA
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 91.579Å b: 117.117Å c: 80.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Purine and pyrimidine pathways as targets in Plasmodium falciparum.
Cassera et al. (2011)
3 more

6 mentions without citation

Heterologous expression of plasmodial proteins for structural studies and functional annotation.
Birkholtz et al. (2008)
5 more