› 1p7m

Solution NMR

SOLUTION STRUCTURE AND BASE PERTURBATION STUDIES REVEAL A NOVEL MODE OF ALKYLATED BASE RECOGNITION BY 3-METHYLADENINE DNA GLYCOSYLASE I

Released:
DOI: 10.2210/pdb1p7m/pdb
PDB entry 1p7m coloured by chain, ensemble of 25 models, front view.
PDB entry 1p7m coloured by chain, ensemble of 25 models, side view.
PDB entry 1p7m coloured by chain, ensemble of 25 models, top view.
Source organism: Escherichia coli
Primary publication:
Solution structure and base perturbation studies reveal a novel mode of alkylated base recognition by 3-methyladenine DNA glycosylase I.
Cao C, Kwon K, Jiang YL, Drohat AC, Stivers JT
J Biol Chem 278 48012-20 (2003)
PMID: 13129925

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alkylated DNA, releasing 3-methyladenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-3-methyladenine glycosylase 1 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P05100 (Residues: 1-187; Coverage: 100%)
Gene names: JW3518, b3549, tag
Sequence domains: Methyladenine glycosylase
Structure domains: Hypothetical protein; domain 2

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand ADK 1 x ADK
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Base excision repair.
Krokan et al. (2013)
3 more

1 mention without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)