› 1p7l

X-ray diffraction
2.5Å resolution

S-Adenosylmethionine synthetase complexed with AMPPNP and Met.

Released:
DOI: 10.2210/pdb1p7l/pdb
PDB entry 1p7l coloured by chain, front view.
PDB entry 1p7l coloured by chain, side view.
PDB entry 1p7l coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of the S-adenosylmethionine synthetase ternary complex: a novel catalytic mechanism of S-adenosylmethionine synthesis from ATP and Met.
Komoto J, Yamada T, Takata Y, Markham GD, Takusagawa F
Biochemistry 43 1821-31 (2004)
PMID: 14967023

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 383 amino acids
Theoretical weight: 41.87 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A817 (Residues: 2-384; Coverage: 100%)
Gene names: JW2909, b2942, metK, metX
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3

Ligands and Environments

6 bound ligands:
Ligand K 4 x K
Ligand MG 8 x MG
Ligand ANP 2 x ANP
Ligand MET 2 x MET
Ligand SAM 2 x SAM
Ligand PPK 2 x PPK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 225.82Å b: 69.13Å c: 118.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.242
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Role of Na+ and K+ in enzyme function.
Page et al. (2006)
8 more

12 mentions without citation

Structure-function relationships in methionine adenosyltransferases.
Markham et al. (2009)
11 more