› 1p4n

X-ray diffraction
1.9Å resolution

Crystal Structure of Weissella viridescens FemX:UDP-MurNAc-pentapeptide complex

Released:
DOI: 10.2210/pdb1p4n/pdb
PDB entry 1p4n coloured by chain, front view.
PDB entry 1p4n coloured by chain, side view.
PDB entry 1p4n coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of Weissella viridescens FemX and its complex with UDP-MurNAc-pentapeptide: insights into FemABX family substrates recognition.
Biarrotte-Sorin S, Maillard AP, Delettré J, Sougakoff W, Arthur M, Mayer C
Structure 12 257-67 (2004)
PMID: 14962386

Function and Biology Details

Reaction catalysed: 
L-alanyl-tRNA(Ala) + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine = tRNA(Ala) + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N(6)-(L-alanyl)-L-lysyl-D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162374 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
UDP-N-acetylmuramoylpentapeptide-lysine N(6)-alanyltransferase Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 38.2 KDa
Source organism: Weissella viridescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EY50 (Residues: 2-336; Coverage: 100%)
Gene name: femX
Sequence domains: FemAB family
Structure domains: Aminopeptidase
UDP-MurNAc-pentapeptide Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 1.15 KDa
Source organism: Staphylococcus aureus

Ligands and Environments

2 bound ligands:
Ligand MG 3 x MG
Ligand GOL 2 x GOL
3 modified residues:
Ligand UMA 1 x UMA
Ligand FGA 1 x FGA
Ligand DAL 2 x DAL

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21
Unit cell:
a: 42.201Å b: 100.61Å c: 46.538Å
α: 90° β: 115.64° γ: 90°
R-values:
R R work R free
0.185 0.172 0.207
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Peptidoglycan structure and architecture.
Vollmer et al. (2008)
17 more

6 mentions without citation

Bacterial GCN5-Related N-Acetyltransferases: From Resistance to Regulation.
Favrot et al. (2016)
5 more