› 1p47

X-ray diffraction
2.2Å resolution

Crystal Structure of tandem Zif268 molecules complexed to DNA

Released:
DOI: 10.2210/pdb1p47/pdb
PDB entry 1p47 coloured by chain, front view.
PDB entry 1p47 coloured by chain, side view.
PDB entry 1p47 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Constraints for zinc finger linker design as inferred from X-ray crystal structure of tandem Zif268-DNA complexes.
Peisach E, Pabo CO
J Mol Biol 330 1-7 (2003)
PMID: 12818197

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114354 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Early growth response protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 10.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08046 (Residues: 333-419; Coverage: 16%)
Gene names: Egr-1, Egr1, Krox-24
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
5'-D(*GP*TP*GP*GP*CP*GP*TP*GP*GP*GP*CP*GP*GP*CP*GP*TP*GP*GP*GP*CP*GP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 22 nucleotides
Theoretical weight: 6.94 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*CP*AP*CP*GP*CP*CP*CP*AP*CP*GP*CP*CP*GP*CP*CP*CP*AP*CP*GP*CP*CP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 22 nucleotides
Theoretical weight: 6.57 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P31
Unit cell:
a: 70.629Å b: 70.629Å c: 99.608Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.214 0.267
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Designing transcription factor architectures for drug discovery.
Blancafort et al. (2004)
3 more

14 mentions without citation

The triple helix: 50 years later, the outcome.
Duca et al. (2008)
13 more