› 1p22

X-ray diffraction
2.95Å resolution

Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity on the SCFbeta-TrCP1 ubiquitin ligase

Released:
DOI: 10.2210/pdb1p22/pdb
PDB entry 1p22 coloured by chain, front view.
PDB entry 1p22 coloured by chain, side view.
PDB entry 1p22 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a beta-TrCP1-Skp1-beta-catenin complex: destruction motif binding and lysine specificity of the SCF(beta-TrCP1) ubiquitin ligase.
Wu G, Xu G, Schulman BA, Jeffrey PD, Harper JW, Pavletich NP
Mol Cell 11 1445-56 (2003)
PMID: 12820959

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-152833 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-box/WD repeat-containing protein 1A Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 49.7 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y297 (Residues: 175-605; Coverage: 71%)
Gene names: BTRC, BTRCP, FBW1A, FBXW1A
Sequence domains:
Structure domains:
S-phase kinase-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 16.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63208 (Residues: 1-163; Coverage: 89%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
Catenin beta-1 Chain: C
Molecule details ›
Chain: C
Length: 26 amino acids
Theoretical weight: 2.9 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P35222 (Residues: 19-44; Coverage: 3%)
Gene names: CTNNB, CTNNB1, OK/SW-cl.35, PRO2286

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P31
Unit cell:
a: 82.6Å b: 82.6Å c: 111.5Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.23 0.286
Expression system: Spodoptera frugiperda

Quick links

Citations

100 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
99 more

133 mentions without citation

Perspective on genes and mutations causing retinitis pigmentosa.
Daiger et al. (2007)
132 more