› 1oyp

X-ray diffraction
2.76Å resolution

Crystal Structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis

Released:
DOI: 10.2210/pdb1oyp/pdb
PDB entry 1oyp coloured by chain, front view.
PDB entry 1oyp coloured by chain, side view.
PDB entry 1oyp coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the phosphorolytic exoribonuclease RNase PH from Bacillus subtilis and implications for its quaternary structure and tRNA binding.
Harlow LS, Kadziola A, Jensen KF, Larsen S
Protein Sci 13 668-77 (2004)
PMID: 14767080

Function and Biology Details

Reaction catalysed: 
tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease PH Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 245 amino acids
Theoretical weight: 26.71 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P28619 (Residues: 1-245; Coverage: 100%)
Gene names: BSU28370, rph
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 51.31Å b: 163.56Å c: 166.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.285 0.306

Quick links

Citations

9 review citations

Messenger RNA turnover in eukaryotes: pathways and enzymes.
Meyer et al. (2004)
8 more

2 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
1 more