› 1ovu

X-ray diffraction
3.1Å resolution

CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL di-Co(II)-DF1-L13A (form I)

Released:
DOI: 10.2210/pdb1ovu/pdb
PDB entry 1ovu coloured by chain, front view.
PDB entry 1ovu coloured by chain, side view.
PDB entry 1ovu coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Response of a designed metalloprotein to changes in metal ion coordination, exogenous ligands, and active site volume determined by X-ray crystallography.
Geremia S, Di Costanzo L, Randaccio L, Engel DE, Lombardi A, Nastri F, DeGrado WF
J Am Chem Soc 127 17266-76 (2005)
PMID: 16332076

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
four-helix bundle model di-Co(II)-DF1-L13A (form I) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 50 amino acids
Theoretical weight: 5.83 KDa
Source organism: Synthetic construct
Expression system: Not provided
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

1 bound ligand:
Ligand CO 7 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: C2221
Unit cell:
a: 89.78Å b: 147.72Å c: 37.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.304
Expression system: Not provided

Quick links

Citations

4 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
3 more

2 mentions without citation

Metal-binding sites are designed to achieve optimal mechanical and signaling properties.
Dutta et al. (2010)
1 more