› 1oty

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142186 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase DeoD-type Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 238 amino acids
Theoretical weight: 25.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABP8 (Residues: 2-239; Coverage: 100%)
Gene names: JW4347, b4384, deoD, pup
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand 6MP 3 x 6MP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6122
Unit cell:
a: 119.97Å b: 119.97Å c: 240.46Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.236
Expression system: Escherichia coli

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Citations

8 review citations

Suicide genes for cancer therapy.
Portsmouth et al. (2007)
7 more