› 1oto

X-ray diffraction
1.96Å resolution

Calcium-binding mutant of the internalin B LRR domain

Released:
DOI: 10.2210/pdb1oto/pdb
PDB entry 1oto coloured by chain, front view.
PDB entry 1oto coloured by chain, side view.
PDB entry 1oto coloured by chain, top view.
Source organism: Listeria monocytogenes
Primary publication:
Characterization of the calcium-binding sites of Listeria monocytogenes InlB.
Marino M, Banerjee M, Copp J, Dramsi S, Chapman T, van der Geer P, Cossart P, Ghosh P
Biochem Biophys Res Commun 316 379-86 (2004)
PMID: 15020228

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Internalin B Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.17 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DQD2 (Residues: 36-248; Coverage: 36%)
Gene names: inlB, lmo0434
Sequence domains:
Structure domains: Ribonuclease Inhibitor

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 43.988Å b: 56.125Å c: 83.414Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.189 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors.
Matsushima et al. (2007)
1 more

1 mention without citation

Design of a binding scaffold based on variable lymphocyte receptors of jawless vertebrates by module engineering.
Lee et al. (2012)