› 1osz

X-ray diffraction
2.1Å resolution

MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN

Released:
DOI: 10.2210/pdb1osz/pdb
PDB entry 1osz coloured by chain, front view.
PDB entry 1osz coloured by chain, side view.
PDB entry 1osz coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands.
Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL
Proc Natl Acad Sci U S A 95 10061-6 (1998)
PMID: 9707600

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135053 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01901 (Residues: 22-295; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 970 Da

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21212
Unit cell:
a: 136.7Å b: 88Å c: 45.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.293
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The structural basis of αβ T-lineage immune recognition: TCR docking topologies, mechanotransduction, and co-receptor function.
Wang et al. (2012)
8 more

6 mentions without citation

Modeling the structure of bound peptide ligands to major histocompatibility complex.
Tong et al. (2004)
5 more