› 1op8

X-ray diffraction
2.5Å resolution

Crystal Structure of Human Granzyme A

Released:
DOI: 10.2210/pdb1op8/pdb
PDB entry 1op8 coloured by chain, front view.
PDB entry 1op8 coloured by chain, side view.
PDB entry 1op8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the apoptosis-inducing human granzyme A dimer.
Hink-Schauer C, Estébanez-Perpiñá E, Kurschus FC, Bode W, Jenne DE
Nat Struct Biol 10 535-40 (2003)
PMID: 12819770

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including fibronectin, type IV collagen and nucleolin. Preferential cleavage: -Arg-|-, -Lys-|- >> -Phe-|- in small molecule substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Granzyme A Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 234 amino acids
Theoretical weight: 25.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12544 (Residues: 29-262; Coverage: 99%)
Gene names: CTLA3, GZMA, HFSP
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand SO4 12 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P1
Unit cell:
a: 49.48Å b: 94.55Å c: 94.87Å
α: 117.12° β: 100.25° γ: 100.12°
R-values:
R R work R free
0.218 0.218 0.284
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Death by a thousand cuts: granzyme pathways of programmed cell death.
Chowdhury et al. (2008)
5 more

5 mentions without citation

Predicting the accuracy of protein-ligand docking on homology models.
Bordogna et al. (2011)
4 more