1omw Summary

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Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and gamma 2 subunits

The structure was published by Lodowski, D.T., Pitcher, J.A., Capel, W.D., Lefkowitz, R.J., and Tesmer, J.J.G., in 2003 in a paper entitled "Keeping G proteins at Bay: A Complex Between G Protein-Coupled Receptor Kinase 2 and G-Beta-Gamma" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.5 Å and deposited in 2003.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely G-protein coupled receptor kinase 2, Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1, and Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-2 subunit.

The molecule most likely forms heterotrimers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A G-protein coupled receptor kinase 2 P21146 (1-689) (ARBK1_BOVIN)search Bos taurussearch 97% 689 89%
B Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1 P62871 (1-340) (GBB1_BOVIN)search Bos taurussearch 100% 340 99%
G Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-2 subunit P63212 (1-68) (GBG2_BOVIN)search Bos taurussearch 94% 74 82%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P21146 (1 - 689) G-protein coupled receptor kinase 2 Bos taurus
P62871 (1 - 340) Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1 Bos taurus
P63212 (1 - 68) Guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-2 subunit Bos taurus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P21146) Regulator of G-protein signaling, RGSsearch, Pleckstrin-homology domain (PH domain)search, Protein kinases, catalytic subunitsearch Regulator of G-protein Signalling 4, domain 2search, Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search, Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)search PF00069: Protein kinase domainsearch, PF00169: PH domainsearch, PF00615: Regulator of G protein signaling domainsearch
B (P62871) WD40-repeatsearch YVTN repeat-like/Quinoprotein amine dehydrogenasesearch PF00400: WD domain, G-beta repeatsearch
G (P63212) Transducin (heterotrimeric G protein), gamma chainsearch G Protein Gi Gamma 2search PF00631: GGL domainsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P21146) protein phosphorylationsearch peptidyl-serine phosphorylationsearch regulation of the force of heart contractionsearch heart developmentsearch negative regulation of the force of heart contraction by chemical signalsearch cardiac muscle contractionsearch receptor internalizationsearch termination of G-protein coupled receptor signaling pathwaysearch signal transductionsearch G-protein coupled receptor internalizationsearch G-protein coupled receptor signaling pathwaysearch desensitization of G-protein coupled receptor protein signaling pathwaysearch negative regulation of striated muscle contractionsearch tachykinin receptor signaling pathwaysearch phosphorylationsearch regulation of signal transductionsearch G-protein coupled acetylcholine receptor signaling pathwaysearch peptidyl-threonine phosphorylationsearch intracellular protein transportsearch G-protein coupled receptor kinase activitysearch beta-adrenergic receptor kinase activitysearch Edg-2 lysophosphatidic acid receptor bindingsearch protein kinase activitysearch protein serine/threonine kinase activitysearch ATP bindingsearch transferase activity, transferring phosphorus-containing groupssearch nucleotide bindingsearch kinase activitysearch transferase activitysearch protein bindingsearch alpha-2A adrenergic receptor bindingsearch membranesearch cytoplasmsearch cytosolsearch
B (P62871) cellular response to prostaglandin E stimulussearch cell proliferationsearch cellular response to catecholamine stimulussearch signal transductionsearch GTP catabolic processsearch sensory perception of tastesearch adenylate cyclase-activating dopamine receptor signaling pathwaysearch phospholipase C-activating G-protein coupled receptor signaling pathwaysearch G-protein coupled receptor signaling pathwaysearch retina development in camera-type eyesearch signal transducer activitysearch GTPase activitysearch protein bindingsearch protein complex bindingsearch GTPase bindingsearch photoreceptor disc membranesearch photoreceptor outer segmentsearch intracellularsearch extracellular vesicular exosomesearch lysosomal membranesearch heterotrimeric G-protein complexsearch membranesearch
G (P63212) adenylate cyclase-activating dopamine receptor signaling pathwaysearch G-protein coupled receptor signaling pathwaysearch cellular response to prostaglandin E stimulussearch cellular response to catecholamine stimulussearch cell proliferationsearch GTP catabolic processsearch signal transductionsearch signal transducer activitysearch G-protein beta-subunit bindingsearch GTPase activitysearch membranesearch heterotrimeric G-protein complexsearch plasma membranesearch extracellular vesicular exosomesearch

Chain InterPro annotation
A GPCR kinasesearch Regulator of G protein signalling domainsearch Protein kinase domainsearch AGC-kinase, C-terminalsearch Pleckstrin homology domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Pleckstrin homology-like domainsearch Regulator of G protein signalling superfamilysearch Protein kinase, ATP binding sitesearch
B G-protein, beta subunitsearch WD40 repeatsearch WD40/YVTN repeat-like-containing domainsearch Guanine nucleotide-binding protein, beta subunitsearch WD40-repeat-containing domainsearch WD40 repeat, conserved sitesearch G-protein beta WD-40 repeatsearch
G G-protein, gamma subunitsearch G-protein gamma-like domainsearch