1oh4

X-ray diffraction
1.35Å resolution

Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.49 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RIK9 (Residues: 505-680; Coverage: 26%)
Gene name: manB
Sequence domains: Carbohydrate binding module 27
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA, GLA
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 31.79Å b: 48.919Å c: 96.421Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.17
Expression system: Escherichia coli BL21(DE3)