› 1ofe

X-ray diffraction
2.45Å resolution

Glutamate Synthase from Synechocystis sp in complex with 2-Oxoglutarate and L-DON at 2.45 Angstrom resolution

Released:
DOI: 10.2210/pdb1ofe/pdb
PDB entry 1ofe coloured by chain, front view.
PDB entry 1ofe coloured by chain, side view.
PDB entry 1ofe coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
The active conformation of glutamate synthase and its binding to ferredoxin.
van den Heuvel RH, Svergun DI, Petoukhov MV, Coda A, Curti B, Ravasio S, Vanoni MA, Mattevi A
J Mol Biol 330 113-28 (2003)
PMID: 12818206

Function and Biology Details

Reaction catalysed: 
(1a) L-glutamate + NH(3) = L-glutamine + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin-dependent glutamate synthase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1520 amino acids
Theoretical weight: 165.71 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P55038 (Residues: 37-1556; Coverage: 98%)
Gene names: gltS, sll1499
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
3 bound ligands:
Ligand F3S 2 x F3S
Ligand AKG 2 x AKG
Ligand ONL 2 x ONL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 122.669Å b: 134.377Å c: 198.774Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.257
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Investigation of intact protein complexes by mass spectrometry.
Heck et al. (2004)
9 more

2 mentions without citation

On the Natural History of Flavin-Based Electron Bifurcation.
Baymann et al. (2018)
1 more