› 1odh

X-ray diffraction
2.85Å resolution

Structure of the GCM domain bound to DNA

Released:
DOI: 10.2210/pdb1odh/pdb
PDB entry 1odh coloured by chain, front view.
PDB entry 1odh coloured by chain, side view.
PDB entry 1odh coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the GCM domain-DNA complex: a DNA-binding domain with a novel fold and mode of target site recognition.
Cohen SX, Moulin M, Hashemolhosseini S, Kilian K, Wegner M, Müller CW
EMBO J 22 1835-45 (2003)
PMID: 12682016

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114302 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Chorion-specific transcription factor GCMa Chain: A
Molecule details ›
Chain: A
Length: 174 amino acids
Theoretical weight: 20.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P70348 (Residues: 1-174; Coverage: 40%)
Gene names: Gcm1, Gcma
Sequence domains: GCM motif protein
Structure domains:
5'-D(*CP*GP*AP*TP*GP*CP*GP*GP*GP*TP *GP*CP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*TP*GP*CP*AP*CP*CP*CP*GP*CP*AP *TP*CP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 41.81Å b: 52.91Å c: 62.97Å
α: 90° β: 103.24° γ: 90°
R-values:
R R work R free
0.219 0.219 0.281
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

8 review citations

NAC transcription factors: structurally distinct, functionally diverse.
Olsen et al. (2005)
7 more

11 mentions without citation

Plant Transcription Factors Involved in Drought and Associated Stresses.
Hrmova et al. (2021)
10 more