› 1ocu

X-ray diffraction
2.3Å resolution

Crystal structure of the yeast PX-domain protein Grd19p (sorting nexin 3) complexed to phosphatidylinosytol-3-phosphate.

Released:
DOI: 10.2210/pdb1ocu/pdb
PDB entry 1ocu coloured by chain, front view.
PDB entry 1ocu coloured by chain, side view.
PDB entry 1ocu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of the yeast Phox homology (PX) domain protein Grd19p complexed to phosphatidylinositol-3-phosphate.
Zhou CZ, Li de La Sierra-Gallay I, Quevillon-Cheruel S, Collinet B, Minard P, Blondeau K, Henckes G, Aufrère R, Leulliot N, Graille M, Sorel I, Savarin P, de la Torre F, Poupon A, Janin J, van Tilbeurgh H
J Biol Chem 278 50371-6 (2003)
PMID: 14514667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170695 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sorting nexin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.88 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08826 (Residues: 1-162; Coverage: 100%)
Gene names: GRD19, SNX3, YOR357C
Sequence domains: PX domain
Structure domains: Phox-like domain

Ligands and Environments

1 bound ligand:
Ligand PIB 2 x PIB
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 31.43Å b: 55.76Å c: 64.75Å
α: 110.75° β: 97.35° γ: 99.49°
R-values:
R R work R free
0.221 0.221 0.256
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Membrane-protein interactions in cell signaling and membrane trafficking.
Cho et al. (2005)
6 more

11 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
10 more