› 1ob8

X-ray diffraction
1.8Å resolution

Holliday Junction Resolving Enzyme

Released:
DOI: 10.2210/pdb1ob8/pdb
PDB entry 1ob8 coloured by chain, front view.
PDB entry 1ob8 coloured by chain, side view.
PDB entry 1ob8 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Substrate recognition and catalysis by the Holliday junction resolving enzyme Hje.
Middleton CL, Parker JL, Richard DJ, White MF, Bond CS
Nucleic Acids Res 32 5442-51 (2004)
PMID: 15479781

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Crossover junction endodeoxyribonuclease Hje Chains: A, B
Molecule details ›
Chains: A, B
Length: 135 amino acids
Theoretical weight: 15.48 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97YX6 (Residues: 1-135; Coverage: 100%)
Gene names: SSO1176, hje
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P65
Unit cell:
a: 90.577Å b: 90.577Å c: 70.92Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.197 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Cruciform structures are a common DNA feature important for regulating biological processes.
Brázda et al. (2011)
5 more

13 mentions without citation

A novel immunity system for bacterial nucleic acid degrading toxins and its recruitment in various eukaryotic and DNA viral systems.
Zhang et al. (2011)
12 more