› 1ob2

X-ray diffraction
3.35Å resolution

E. coli elongation factor EF-Tu complexed with the antibiotic kirromycin, a GTP analog, and Phe-tRNA

Released:
DOI: 10.2210/pdb1ob2/pdb
PDB entry 1ob2 coloured by chain, front view.
PDB entry 1ob2 coloured by chain, side view.
PDB entry 1ob2 coloured by chain, top view.
Source organisms:
Entry authors: Kristensen O, Nissen P, Nyborg J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143217 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Elongation factor Tu 2 Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 43.22 KDa
Source organism: Escherichia coli BL21
UniProt:
  • Canonical: P0CE48 (Residues: 2-394; Coverage: 100%)
Gene names: JW3943, b3980, tufB
Sequence domains:
Structure domains:
TRANSFER-RNA, PHE Chain: B
Molecule details ›
Chain: B
Length: 77 nucleotides
Theoretical weight: 25.01 KDa
Source organism: Saccharomyces cerevisiae
Sequence domains: tRNA

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
3 bound ligands:
Ligand KIR 1 x KIR
Ligand GNP 1 x GNP
Ligand MG 1 x MG
11 modified residues:
Ligand 2MG 1 x 2MG
Ligand H2U 2 x H2U
Ligand M2G 1 x M2G
Ligand OMC 1 x OMC
Ligand OMG 1 x OMG
Ligand YG 1 x YG
Ligand PSU 2 x PSU
Ligand 5MC 2 x 5MC
Ligand 7MG 1 x 7MG
Ligand 1MA 1 x 1MA
Ligand PHA 1 x PHA

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P4132
Unit cell:
a: 196.41Å b: 196.41Å c: 196.41Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.265

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