› 1oaj

X-ray diffraction
1.73Å resolution

Active site copper and zinc ions modulate the quaternary structure of prokaryotic Cu,Zn superoxide dismutase

Released:
DOI: 10.2210/pdb1oaj/pdb
PDB entry 1oaj coloured by chain, front view.
PDB entry 1oaj coloured by chain, side view.
PDB entry 1oaj coloured by chain, top view.
Source organism: Photobacterium leiognathi subsp. leiognathi
Primary publication:
Active-site copper and zinc ions modulate the quaternary structure of prokaryotic Cu,Zn superoxide dismutase.
Cioni P, Pesce A, Morozzo della Rocca B, Castelli S, Falconi M, Parrilli L, Bolognesi M, Strambini G, Desideri A
J Mol Biol 326 1351-60 (2003)
PMID: 12595249

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Cu-Zn] Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 15.8 KDa
Source organism: Photobacterium leiognathi subsp. leiognathi
Expression system: Escherichia coli
UniProt:
  • Canonical: P00446 (Residues: 23-173; Coverage: 100%)
Gene name: sodC
Sequence domains: Copper/zinc superoxide dismutase (SODC)
Structure domains: Superoxide dismutase, copper/zinc binding domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: R32
Unit cell:
a: 85.9Å b: 85.9Å c: 98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.183 0.231
Expression system: Escherichia coli

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Citations

8 citation in other articles

The subunit interfaces of weakly associated homodimeric proteins.
Dey et al. (2010)
7 more