› 1oah

X-ray diffraction
2.3Å resolution

Cytochrome c Nitrite Reductase from Desulfovibrio desulfuricans ATCC 27774: The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA).

Released:
DOI: 10.2210/pdb1oah/pdb
PDB entry 1oah coloured by chain, front view.
PDB entry 1oah coloured by chain, side view.
PDB entry 1oah coloured by chain, top view.
Source organism: Desulfovibrio desulfuricans
Primary publication:
Cytochrome c nitrite reductase from Desulfovibrio desulfuricans ATCC 27774. The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA).
Cunha CA, Macieira S, Dias JM, Almeida G, Goncalves LL, Costa C, Lampreia J, Huber R, Moura JJ, Moura I, Romão MJ
J Biol Chem 278 17455-65 (2003)
PMID: 12618432

Function and Biology Details

Reaction catalysed: 
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nitrite reductase (cytochrome; ammonia-forming) Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 59.23 KDa
Source organism: Desulfovibrio desulfuricans
UniProt:
  • Canonical: Q8VNU2 (Residues: 1-514; Coverage: 100%)
Gene name: nrfA
Sequence domains: Cytochrome c552
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 10 x HEC
3 bound ligands:
Ligand CA 4 x CA
Ligand CL 4 x CL
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 78.94Å b: 104.6Å c: 143.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.224

Quick links

Citations

9 review citations

Respiration of metal (hydr)oxides by Shewanella and Geobacter: a key role for multihaem c-type cytochromes.
Shi et al. (2007)
8 more

6 mentions without citation

X-ray structure of the membrane-bound cytochrome c quinol dehydrogenase NrfH reveals novel haem coordination.
Rodrigues et al. (2006)
5 more