› 1o9i

X-ray diffraction
1.33Å resolution

Crystal structure of the Y42F mutant of manganese catalase from Lactobacillus plantarum at 1.33A resolution

Released:
DOI: 10.2210/pdb1o9i/pdb
PDB entry 1o9i coloured by chain, front view.
PDB entry 1o9i coloured by chain, side view.
PDB entry 1o9i coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum
Primary publication:
Outer sphere mutagenesis of Lactobacillus plantarum manganese catalase disrupts the cluster core. Mechanistic implications.
Whittaker MM, Barynin VV, Igarashi T, Whittaker JW
Eur J Biochem 270 1102-16 (2003)
PMID: 12631270

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Manganese catalase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 266 amino acids
Theoretical weight: 29.76 KDa
Source organism: Lactiplantibacillus plantarum
Expression system: Lactiplantibacillus plantarum
UniProt:
  • Canonical: P60355 (Residues: 1-266; Coverage: 100%)
Sequence domains: Manganese containing catalase
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MN3 12 x MN3
Ligand CA 6 x CA
Ligand O 6 x O
Ligand MES 5 x MES
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 73.49Å b: 95.27Å c: 105Å
α: 90° β: 106.55° γ: 90°
R-values:
R R work R free
0.117 0.115 0.145
Expression system: Lactiplantibacillus plantarum

Quick links

Citations

9 review citations

Evolution of catalases from bacteria to humans.
Zamocky et al. (2008)
8 more

1 mention without citation

How long is a piece of loop?
Choi et al. (2013)