› 1o87

X-ray diffraction
2.1Å resolution

A new MgGDP complex of the Ffh NG domain

Released:
DOI: 10.2210/pdb1o87/pdb
PDB entry 1o87 coloured by chain, front view.
PDB entry 1o87 coloured by chain, side view.
PDB entry 1o87 coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Novel protein and Mg2+ configurations in the Mg2+GDP complex of the SRP GTPase ffh.
Focia PJ, Alam H, Lu T, Ramirez UD, Freymann DM
Proteins 54 222-30 (2004)
PMID: 14696184

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 32.57 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: O07347 (Residues: 1-297; Coverage: 69%)
Gene name: ffh
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CL 1 x CL
Ligand FMT 5 x FMT
Ligand GDP 2 x GDP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 55.415Å b: 113.631Å c: 61.382Å
α: 90° β: 111.76° γ: 90°
R-values:
R R work R free
0.197 0.195 0.237
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Heterodimeric GTPase core of the SRP targeting complex.
Focia et al. (2004)
6 more

3 mentions without citation

Mechanistic insights into the three steps of poly(ADP-ribosylation) reversal.
Rack et al. (2021)
2 more