Function and Biology

Crystal structure of Ribonuclease III (TM1102) from Thermotoga maritima at 2.0 A resolution

Source organism: Thermotoga maritima
Biochemical function: metal ion binding
Biological process: pre-miRNA processing
Cellular component: RISC complex

EC 3.1.26.3: Ribonuclease III

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester.
Systematic name:
-
Alternative Name(s):
  • RNase III
  • Ribonuclease 3

Sequence families

Pfam Protein families (Pfam)
PF00035
Domain description: Double-stranded RNA binding motif
Occurring in:
  1. Ribonuclease 3
The deposited structure of PDB entry 1o0w contains 2 copies of Pfam domain PF00035 (Double-stranded RNA binding motif) in Ribonuclease 3. Showing 1 copy in chain B.

PF14622
Domain description: Ribonuclease-III-like
Occurring in:
  1. Ribonuclease 3
The deposited structure of PDB entry 1o0w contains 2 copies of Pfam domain PF14622 (Ribonuclease-III-like) in Ribonuclease 3. Showing 1 copy in chain B.

InterPro InterPro annotations
IPR000999
Domain description: Ribonuclease III domain
Occurring in:
  1. Ribonuclease 3
IPR014720
Domain description: Double-stranded RNA-binding domain
Occurring in:
  1. Ribonuclease 3
IPR011907
Domain description: Ribonuclease III
Occurring in:
  1. Ribonuclease 3
IPR036389
Domain description: Ribonuclease III, endonuclease domain superfamily
Occurring in:
  1. Ribonuclease 3

Structure domains

CATH CATH domains
3.30.160.20
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Double Stranded RNA Binding Domain
Homology: Double Stranded RNA Binding Domain
Occurring in:
  1. Ribonuclease 3
The deposited structure of PDB entry 1o0w contains 2 copies of CATH domain 3.30.160.20 (Double Stranded RNA Binding Domain) in Ribonuclease 3. Showing 1 copy in chain B.
1.10.1520.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Ribonuclease iii, N-terminal Endonuclease Domain; Chain A
Homology: Ribonuclease III domain
Occurring in:
  1. Ribonuclease 3
The deposited structure of PDB entry 1o0w contains 2 copies of CATH domain 1.10.1520.10 (Ribonuclease iii, N-terminal Endonuclease Domain; Chain A) in Ribonuclease 3. Showing 1 copy in chain B.
SCOP SCOP annotations
54769
Class: Alpha and beta proteins (a+b)
Fold: dsRBD-like
Superfamily: dsRNA-binding domain-like
Occurring in:
  1. Ribonuclease 3
The deposited structure of PDB entry 1o0w contains 2 copies of SCOP domain 54769 (Double-stranded RNA-binding domain (dsRBD)) in Ribonuclease 3. Showing 1 copy in chain B.
69066
Class: All alpha proteins
Fold: RNase III domain-like
Superfamily: RNase III domain-like
Occurring in:
  1. Ribonuclease 3
The deposited structure of PDB entry 1o0w contains 2 copies of SCOP domain 69066 (RNase III catalytic domain-like) in Ribonuclease 3. Showing 1 copy in chain B.