› 1o0t

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF A COLD ADAPTED ALKALINE PROTEASE FROM PSEUDOMONAS TAC II 18, CO-CRYSTALLIZED WITH 5 mM EDTA (5 DAYS)

Released:
DOI: 10.2210/pdb1o0t/pdb
PDB entry 1o0t coloured by chain, front view.
PDB entry 1o0t coloured by chain, side view.
PDB entry 1o0t coloured by chain, top view.
Source organism: Pseudomonas sp. 'TAC II 18'
Primary publication:
Probing the role of divalent metal ions in a bacterial psychrophilic metalloprotease: binding studies of an enzyme in the crystalline state by x-ray crystallography.
Ravaud S, Gouet P, Haser R, Aghajari N
J Bacteriol 185 4195-203 (2003)
PMID: 12837794

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of bonds with hydrophobic residues in P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase metallopeptidase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 463 amino acids
Theoretical weight: 48.73 KDa
Source organism: Pseudomonas sp. 'TAC II 18'
UniProt:
  • Canonical: O69771 (Residues: 18-480; Coverage: 97%)
Gene name: PAPRA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 7 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: R3
Unit cell:
a: 185.7Å b: 185.7Å c: 38Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.167 0.218

Quick links

Citations

2 review citations

Proteases from psychrotrophs: an overview.
Kasana RC. (2010)
1 more

1 mention without citation

Probing the role of divalent metal ions in a bacterial psychrophilic metalloprotease: binding studies of an enzyme in the crystalline state by x-ray crystallography.
Ravaud et al. (2003)