› 1nzl

X-ray diffraction
1.9Å resolution

Crystal Structure of Src SH2 domain bound to doubly phosphorylated peptide PQpYEpYIPI

Released:
DOI: 10.2210/pdb1nzl/pdb
PDB entry 1nzl coloured by chain, front view.
PDB entry 1nzl coloured by chain, side view.
PDB entry 1nzl coloured by chain, top view.
Source organism: Rous sarcoma virus (strain Schmidt-Ruppin)
Primary publication:
Structural and thermodynamic basis for the interaction of the Src SH2 domain with the activated form of the PDGF beta-receptor.
Lubman OY, Waksman G
J Mol Biol 328 655-68 (2003)
PMID: 12706723

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132659 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase transforming protein Src Chains: A, B
Molecule details ›
Chains: A, B
Length: 103 amino acids
Theoretical weight: 11.84 KDa
Source organism: Rous sarcoma virus (strain Schmidt-Ruppin)
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00524 (Residues: 145-247; Coverage: 20%)
Gene name: V-SRC
Sequence domains: SH2 domain
Structure domains: SH2 domain
Doubly phosphorylated peptide ligand (PQpYEpYIPI) Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 1.14 KDa
Source organism: Rous sarcoma virus (strain Schmidt-Ruppin)
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand PG4 1 x PG4
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 100.321Å b: 68.455Å c: 29.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.265
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

1 review citation

SH2 Domain Binding: Diverse FLVRs of Partnership.
Jaber Chehayeb et al. (2020)
9 other articles

1 mention without citation

'Hot' macromolecular crystals.
Koclega et al. (2009)