1nx0 Summary


Structure of Calpain Domain 6 in Complex with Calpastatin DIC

The structure was published by Todd, B., Moore, D., Deivanayagam, C.C.S., et al., Maki, M., Wang, K.K.W., and Narayana, S.V.L., in 2003 in a paper entitled "A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.3 Å and deposited in 2003.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely Calcium-dependent protease, small subunit, Calpastatin, and Small molecule inhibitor.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heteropentamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calcium-dependent protease, small subunit P04574 (94-266) (CPNS1_PIG)search Sus scrofasearch < 90% 173 100%
B Calcium-dependent protease, small subunit P04574 (94-266) (CPNS1_PIG)search Sus scrofasearch < 90% 173 100%
C Calpastatin P12675 (231-241) (ICAL_PIG)search Sus scrofasearch < 90% 12 100%
D Calpastatin P12675 (231-241) (ICAL_PIG)search Sus scrofasearch < 90% 12 100%
E Small molecule inhibitor Not available
Synthetic Not available 5 100%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P04574 (94 - 266) Calcium-dependent protease, small subunit Sus scrofa
P12675 (231 - 241) Calpastatin Sus scrofa

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B Penta-EF-hand proteinssearch EF-handsearch EF-hand domainsearch, EF-hand domain pairsearch
C, D Calpain inhibitorsearch

Chain ID Molecular function (GO) Biological process (GO)
A, B (P04574) calcium ion bindingsearch proteolysissearch

Chain InterPro annotation
A, B EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch Calpain small subunit 1search
C, D