› 1nvt

X-ray diffraction
2.35Å resolution

Crystal structure of Shikimate Dehydrogenase (AROE or MJ1084) in complex with NADP+

Released:
DOI: 10.2210/pdb1nvt/pdb
PDB entry 1nvt coloured by chain, front view.
PDB entry 1nvt coloured by chain, side view.
PDB entry 1nvt coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of shikimate 5-dehydrogenase (SDH) bound to NADP: insights into function and evolution.
Padyana AK, Burley SK
Structure 11 1005-13 (2003)
PMID: 12906831

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase (NADP(+)) Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.33 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58484 (Residues: 1-282; Coverage: 100%)
Gene names: MJ1084, aroE
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 74.65Å b: 75.62Å c: 118.79Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.26
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The shikimate dehydrogenase family: functional diversity within a conserved structural and mechanistic framework.
Peek et al. (2015)
1 more

7 mentions without citation

High-throughput computational and experimental techniques in structural genomics.
Chance et al. (2004)
6 more