› 1nuf

X-ray diffraction
2.7Å resolution

Role of Calcium Ions in the Activation and Activity of the Transglutaminase 3 Enzyme

Released:
DOI: 10.2210/pdb1nuf/pdb
PDB entry 1nuf coloured by chain, front view.
PDB entry 1nuf coloured by chain, side view.
PDB entry 1nuf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Roles of calcium ions in the activation and activity of the transglutaminase 3 enzyme.
Ahvazi B, Boeshans KM, Idler W, Baxa U, Steinert PM
J Biol Chem 278 23834-41 (2003)
PMID: 12679341

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein-glutamine gamma-glutamyltransferase E Chain: A
Molecule details ›
Chain: A
Length: 692 amino acids
Theoretical weight: 76.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q08188 (Residues: 2-693; Coverage: 100%)
Gene name: TGM3
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 4 x CL
Ligand BR 4 x BR
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 57.74Å b: 115.84Å c: 61.96Å
α: 90° β: 94.14° γ: 90°
R-values:
R R work R free
0.186 0.186 0.254
Expression system: Spodoptera frugiperda

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Citations

12 review citations

Oxidative stress in aging human skin.
Rinnerthaler et al. (2015)
11 more

1 mention without citation

Structure functional insights into calcium binding during the activation of coagulation factor XIII A.
Singh et al. (2019)