› 1nmb

X-ray diffraction
2.2Å resolution

THE STRUCTURE OF A COMPLEX BETWEEN THE NC10 ANTIBODY AND INFLUENZA VIRUS NEURAMINIDASE AND COMPARISON WITH THE OVERLAPPING BINDING SITE OF THE NC41 ANTIBODY

Released:
DOI: 10.2210/pdb1nmb/pdb
PDB entry 1nmb coloured by chain, front view.
PDB entry 1nmb coloured by chain, side view.
PDB entry 1nmb coloured by chain, top view.
Source organisms:
Primary publication:
The structure of a complex between the NC10 antibody and influenza virus neuraminidase and comparison with the overlapping binding site of the NC41 antibody.
Malby RL, Tulip WR, Harley VR, McKimm-Breschkin JL, Laver WG, Webster RG, Colman PM
Structure 2 733-46 (1994)
PMID: 7994573

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209251 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Neuraminidase Chain: N
Molecule details ›
Chain: N
Length: 470 amino acids
Theoretical weight: 52.44 KDa
Source organism: Influenza A virus
UniProt:
  • Canonical: P05803 (Residues: 1-470; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase
FAB NC10 Chain: L
Molecule details ›
Chain: L
Length: 109 amino acids
Theoretical weight: 12.13 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
FAB NC10 Chain: H
Molecule details ›
Chain: H
Length: 122 amino acids
Theoretical weight: 13.42 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 169.4Å b: 169.4Å c: 156.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 not available

Quick links

Citations

18 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
17 more

25 mentions without citation

Understanding the human antibody repertoire.
Rees AR. (2020)
24 more