› 1nks

X-ray diffraction
2.57Å resolution

ADENYLATE KINASE FROM SULFOLOBUS ACIDOCALDARIUS

Released:
DOI: 10.2210/pdb1nks/pdb
PDB entry 1nks coloured by chain, front view.
PDB entry 1nks coloured by chain, side view.
PDB entry 1nks coloured by chain, top view.
Source organism: Sulfolobus acidocaldarius
Primary publication:
The structure of a trimeric archaeal adenylate kinase.
Vonrhein C, Bönisch H, Schäfer G, Schulz GE
J Mol Biol 282 167-79 (1998)
PMID: 9733648

Function and Biology Details

Reaction catalysed: 
ATP + AMP = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-152770 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 194 amino acids
Theoretical weight: 21.14 KDa
Source organism: Sulfolobus acidocaldarius
Expression system: Escherichia coli
UniProt:
  • Canonical: P35028 (Residues: 1-194; Coverage: 100%)
Gene names: Saci_0573, adk, adkA
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand AMP 3 x AMP
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 73.51Å b: 145.69Å c: 172.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.164 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability.
Vieille et al. (2001)
1 more

9 mentions without citation

Zipping and unzipping of adenylate kinase: atomistic insights into the ensemble of open<-->closed transitions.
Beckstein et al. (2009)
8 more