› 1nkn

X-ray diffraction
2.5Å resolution

VISUALIZING AN UNSTABLE COILED COIL: THE CRYSTAL STRUCTURE OF AN N-TERMINAL SEGMENT OF THE SCALLOP MYOSIN ROD

Released:
DOI: 10.2210/pdb1nkn/pdb
PDB entry 1nkn coloured by chain, front view.
PDB entry 1nkn coloured by chain, side view.
PDB entry 1nkn coloured by chain, top view.
Source organisms:
Primary publication:
Visualization of an unstable coiled coil from the scallop myosin rod.
Li Y, Brown JH, Reshetnikova L, Blazsek A, Farkas L, Nyitray L, Cohen C
Nature 424 341-5 (2003)
PMID: 12867988

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136517 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General control transcription factor GCN4; Myosin heavy chain Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 89 amino acids
Theoretical weight: 10.56 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q17042 (Residues: 838-890; Coverage: 3%)
  • Canonical: P03069 (Residues: 250-281; Coverage: 11%)
Gene names: AAS101, AAS3, ARG9, GCN4, YEL009C
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 54.034Å b: 73.303Å c: 102.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.244 0.289
Expression system: Escherichia coli

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Citations

9 review citations

Intermediate filaments: molecular structure, assembly mechanism, and integration into functionally distinct intracellular Scaffolds.
Herrmann et al. (2004)
8 more

5 mentions without citation

The elastic properties of the structurally characterized myosin II S2 subdomain: a molecular dynamics and normal mode analysis.
Adamovic et al. (2008)
4 more