› 1njf

X-ray diffraction
2.3Å resolution

Nucleotide bound form of an isolated E. coli clamp loader gamma subunit

Released:
DOI: 10.2210/pdb1njf/pdb
PDB entry 1njf coloured by chain, front view.
PDB entry 1njf coloured by chain, side view.
PDB entry 1njf coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit.
Podobnik M, Weitze TF, O'Donnell M, Kuriyan J
Structure 11 253-63 (2003)
PMID: 12623013

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit tau Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 250 amino acids
Theoretical weight: 27.5 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06710 (Residues: 1-243; Coverage: 38%)
Gene names: JW0459, b0470, dnaX, dnaZ, dnaZX
Sequence domains: DNA polymerase III, delta subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand AGS 3 x AGS
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 71.708Å b: 113.347Å c: 121.355Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Cellular DNA replicases: components and dynamics at the replication fork.
Johnson et al. (2005)
8 more

4 mentions without citation

Docking protein domains in contact space.
Lise et al. (2006)
3 more