› 1nh6

X-ray diffraction
2.05Å resolution

Structure of S. marcescens chitinase A, E315L, complex with hexasaccharide

Released:
DOI: 10.2210/pdb1nh6/pdb
PDB entry 1nh6 coloured by chain, front view.
PDB entry 1nh6 coloured by chain, side view.
PDB entry 1nh6 coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Family 18 chitinase-oligosaccharide substrate interaction: subsite preference and anomer selectivity of Serratia marcescens chitinase A.
Aronson NN, Halloran BA, Alexyev MF, Amable L, Madura JD, Pasupulati L, Worth C, Van Roey P
Biochem J 376 87-95 (2003)
PMID: 12932195

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase A Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 58.65 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07254 (Residues: 24-563; Coverage: 100%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I222
Unit cell:
a: 78.1Å b: 135.23Å c: 188.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Hydrophilic interaction chromatography.
Hemström et al. (2006)
5 more

6 mentions without citation

Molecular characterization of a novel chitinase CmChi1 from Chitinolyticbacter meiyuanensis SYBC-H1 and its use in N-acetyl-d-glucosamine production.
Zhang et al. (2018)
5 more