› 1nf9

X-ray diffraction
1.5Å resolution

Crystal Structure of PhzD protein from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb1nf9/pdb
PDB entry 1nf9 coloured by chain, front view.
PDB entry 1nf9 coloured by chain, side view.
PDB entry 1nf9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure and mechanism of Pseudomonas aeruginosa PhzD, an isochorismatase from the phenazine biosynthetic pathway.
Parsons JF, Calabrese K, Eisenstein E, Ladner JE
Biochemistry 42 5684-93 (2003)
PMID: 12741825

Function and Biology Details

Reaction catalysed: 
(2S)-2-amino-4-deoxychorismate + H(2)O = (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylate + pyruvate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenazine biosynthesis protein PhzD1 Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.22 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DPB9 (Residues: 1-207; Coverage: 100%)
Gene names: PA4213, phzD, phzD1
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

2 bound ligands:
Ligand BOG 1 x BOG
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 68.85Å b: 77.03Å c: 82.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.155 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Phenazine compounds in fluorescent Pseudomonas spp. biosynthesis and regulation.
Mavrodi et al. (2006)
10 more

8 mentions without citation

Structure-based Druggability Assessment of Anti-virulence Targets from Pseudomonas aeruginosa.
Froes et al. (2019)
7 more