1nej Summary

pdbe.org/1nej
spacer

Crystalline Human Carbonmonoxy Hemoglobin S (Liganded Sickle Cell Hemoglobin) Exhibits The R2 Quaternary State At Neutral pH In The Presence Of Polyethylene Glycol: The 2.1 Angstrom Resolution Crystal Structure

The structure was published by Patskovska, L.N., Patskovsky, Y.V., Almo, S.C., and Hirsch, R.E., in 2005 in a paper entitled "COHbC and COHbS crystallize in the R2 quaternary state at neutral pH in the presence of PEG 4000." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.1 Å and deposited in 2002.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Hemoglobin alpha chain and Hemoglobin beta chain.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Hemoglobin alpha chain P69905 (2-142) (HBA_HUMAN)search Homo sapienssearch 98% 141 100%
C Hemoglobin alpha chain P69905 (2-142) (HBA_HUMAN)search Homo sapienssearch 98% 141 100%
B Hemoglobin beta chain P68871 (2-147) (HBB_HUMAN)search Homo sapienssearch 98% 146 100%
D Hemoglobin beta chain P68871 (2-147) (HBB_HUMAN)search Homo sapienssearch 98% 146 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P69905 (2 - 142) Hemoglobin alpha chain Homo sapiens
P68871 (2 - 147) Hemoglobin beta chain Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P69905) Globinssearch Globinssearch PF00042: Globinsearch
B, D (P68871) Globinssearch Globinssearch PF00042: Globinsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, C (P69905) iron ion bindingsearch heme bindingsearch protein bindingsearch oxygen bindingsearch haptoglobin bindingsearch metal ion bindingsearch oxygen transporter activitysearch peroxidase activitysearch oxygen transportsearch hydrogen peroxide catabolic processsearch bicarbonate transportsearch small molecule metabolic processsearch transportsearch response to hydrogen peroxidesearch positive regulation of cell deathsearch oxidation-reduction processsearch receptor-mediated endocytosissearch protein heterooligomerizationsearch cytosolsearch hemoglobin complexsearch extracellular vesicular exosomesearch extracellular regionsearch endocytic vesicle lumensearch membranesearch haptoglobin-hemoglobin complexsearch blood microparticlesearch cytosolic small ribosomal subunitsearch
B, D (P68871) heme bindingsearch oxygen bindingsearch oxygen transporter activitysearch peroxidase activitysearch protein bindingsearch hemoglobin bindingsearch iron ion bindingsearch metal ion bindingsearch haptoglobin bindingsearch bicarbonate transportsearch renal absorptionsearch receptor-mediated endocytosissearch regulation of blood vessel sizesearch protein heterooligomerizationsearch blood coagulationsearch regulation of blood pressuresearch oxygen transportsearch platelet aggregationsearch small molecule metabolic processsearch response to hydrogen peroxidesearch positive regulation of nitric oxide biosynthetic processsearch nitric oxide transportsearch oxidation-reduction processsearch positive regulation of cell deathsearch hydrogen peroxide catabolic processsearch transportsearch endocytic vesicle lumensearch cytosolsearch extracellular regionsearch hemoglobin complexsearch haptoglobin-hemoglobin complexsearch extracellular vesicular exosomesearch blood microparticlesearch

Chain InterPro annotation
A, C Globinsearch Haemoglobin, alphasearch Haemoglobin, pisearch Globin-likesearch Globin, structural domainsearch
B, D Globinsearch Haemoglobin, betasearch Globin-likesearch Globin, structural domainsearch