› 1neg

X-ray diffraction
2.3Å resolution

Crystal Structure Analysis of N-and C-terminal labeled SH3-domain of alpha-Chicken Spectrin

Released:
DOI: 10.2210/pdb1neg/pdb
PDB entry 1neg coloured by chain, front view.
PDB entry 1neg coloured by chain, side view.
PDB entry 1neg coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Rapid purification and crystal structure analysis of a small protein carrying two terminal affinity tags.
Mueller U, Büssow K, Diehl A, Bartl FJ, Niesen FH, Nyarsik L, Heinemann U
J Struct Funct Genomics 4 217-25 (2003)
PMID: 15185962

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin alpha chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.64 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07751 (Residues: 965-1029; Coverage: 3%)
Gene names: SPTA2, SPTAN1
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

1 bound ligand:
Ligand AZI 1 x AZI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 41.865Å b: 41.865Å c: 92.865Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.249
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Structural genomics of human proteins--target selection and generation of a public catalogue of expression clones.
Büssow et al. (2005)
2 more

1 mention without citation

The role of immune cell subpopulations in the growth and rejection of TC-1/A9 tumors in novel mouse strains differing in the H2-D haplotype and NKC domain.
Indrová et al. (2018)