› 1ncl

X-ray diffraction
2.2Å resolution

THERMAL STABILITY OF HEXAMERIC AND TETRAMERIC NUCLEOSIDE, DIPHOSPHATE KINASES

Released:
DOI: 10.2210/pdb1ncl/pdb
PDB entry 1ncl coloured by chain, front view.
PDB entry 1ncl coloured by chain, side view.
PDB entry 1ncl coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
Thermal stability of hexameric and tetrameric nucleoside diphosphate kinases. Effect of subunit interaction.
Giartosio A, Erent M, Cervoni L, Moréra S, Janin J, Konrad M, Lascu I
J Biol Chem 271 17845-51 (1996)
PMID: 8663370

Function and Biology Details

Reaction catalysed: 
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoside diphosphate kinase, cytosolic Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.21 KDa
Source organism: Dictyostelium discoideum
Expression system: Escherichia coli
UniProt:
  • Canonical: P22887 (Residues: 6-155; Coverage: 97%)
Gene names: DDB_G0273069, DDB_G0273805, gip17, ndkB, ndkC-1, ndkC-2
Sequence domains: Nucleoside diphosphate kinase
Structure domains: Nucleoside diphosphate kinase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE
Spacegroup: P6322
Unit cell:
a: 74.3Å b: 74.3Å c: 105.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Escherichia coli

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Citations

5 review citations

Dictyostelium discoideum--a model for many reasons.
Annesley et al. (2009)
4 more