› 1nat

X-ray diffraction
2.45Å resolution

CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS

Released:
DOI: 10.2210/pdb1nat/pdb
PDB entry 1nat coloured by chain, front view.
PDB entry 1nat coloured by chain, side view.
PDB entry 1nat coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis.
Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI
Biochemistry 36 12739-45 (1997)
PMID: 9335530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sporulation initiation phosphotransferase F Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.24 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06628 (Residues: 1-124; Coverage: 100%)
Gene names: BSU37130, spo0F
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P42212
Unit cell:
a: 58.5Å b: 58.5Å c: 85.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 not available
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
3 more

7 mentions without citation

A common dimerization interface in bacterial response regulators KdpE and TorR.
Toro-Roman et al. (2005)
6 more