› 1n9b

X-ray diffraction
0.9Å resolution

Ultrahigh resolution structure of a class A beta-lactamase: On the mechanism and specificity of the extended-spectrum SHV-2 enzyme

Released:
DOI: 10.2210/pdb1n9b/pdb
PDB entry 1n9b coloured by chain, front view.
PDB entry 1n9b coloured by chain, side view.
PDB entry 1n9b coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Ultrahigh resolution structure of a class A beta-lactamase: on the mechanism and specificity of the extended-spectrum SHV-2 enzyme.
Nukaga M, Mayama K, Hujer AM, Bonomo RA, Knox JR
J Mol Biol 328 289-301 (2003)
PMID: 12684014

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase SHV-3 Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 28.94 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli str. K-12 substr. DH10B
UniProt:
  • Canonical: P30896 (Residues: 22-286; Coverage: 100%)
Gene names: bla, shv3
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand MA4 2 x MA4
Ligand EPE 1 x EPE
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P212121
Unit cell:
a: 49.74Å b: 55.58Å c: 83.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.125 0.15
Expression system: Escherichia coli str. K-12 substr. DH10B

Quick links

Citations

13 review citations

The new beta-lactamases.
Jacoby et al. (2005)
12 more

15 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
14 more