› 1n3e

X-ray diffraction
2.5Å resolution

Crystal structure of I-CreI bound to a palindromic DNA sequence I (palindrome of left side of wildtype DNA target sequence)

Released:
DOI: 10.2210/pdb1n3e/pdb
PDB entry 1n3e coloured by chain, front view.
PDB entry 1n3e coloured by chain, side view.
PDB entry 1n3e coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Flexible DNA target site recognition by divergent homing endonuclease isoschizomers I-CreI and I-MsoI.
Chevalier B, Turmel M, Lemieux C, Monnat RJ, Stoddard BL
J Mol Biol 329 253-69 (2003)
PMID: 12758074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114226 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 163 amino acids
Theoretical weight: 18.72 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 1-163; Coverage: 100%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*C)-3' Chains: C, E, I, K
Molecule details ›
Chains: C, E, I, K
Length: 14 nucleotides
Theoretical weight: 4.27 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided
5'-D(P*GP*AP*CP*GP*TP*TP*TP*TP*CP*G)-3' Chains: D, F, J, L
Molecule details ›
Chains: D, F, J, L
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 46.73Å b: 68.44Å c: 301.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
Silva et al. (2011)
8 more